This　study　aimed　at　assessing　the　occurrence　of　twelve　mycotoxins,　including　deoxynivalenol　(DON)　and　its　derivatives,　3-　and　15-acetyl-deoxynivalenol　(3-ADON　and　15-ADON),　nivalenol　(NIV),　zearalenone　(ZEN),　fumonisins　(FB1,　FB(2　)and　FB3)　and　aflatoxins　(AFB(1),　AFB(2),　AFG(1)　and　AFG(2))　in　338　unprocessed　wheat　samples　harvested　in　2018　from　China　by　high-performance　liquid　chromatography-tandem　mass　spectrometry　(HPLCMS/MS).　DON　was　found　to　be　the　most　predominant　and　abundant　mycotoxins　followed　by　3-ADON,　15-ADON,　NIV,　FB1,　ZEN,　FB3,　FB2,　AFB(1),　AFB(2),　AFG(2)　and　AFG(1).　Samples　from　Hubei　and　Anhui　provinces　that　situated　the　Yangtze　River　recorded　a　higher　positive　rate　for　Fusarium　mycotoxins.　The　positive　rate　and　average　concentration　of　fumonisins　in　wheat　samples　are　relatively　low　while　the　co-occurrence　of　fumonisins　as　well　as　DONs　and　its　derivatives　3-ADON　and　15-ADON,　ZEN　and/or　NIV　was　found　to　be　37.6%.　Moreover,　compared　with　wheat　samples　in　2017,　the　combined　levels　of　DON　and　its　derivatives,　3-ADON　and　15-ADON　in　wheat　samples　harvested　in　the　year　of　2018　is　higher　with　the　incidence　of　co-occurrence　of　DON　+　3-ADON　+　15-ADON　significantly　raised　which　may　due　to　the　increased　precipitation　weather　conditions.　These　findings　suggest　the　necessity　of　monitoring　contamination　of　Fusarium　mycotoxins　and　further　raise　concerns　as　to　DON　+　3ADON　+　15-ADON　co-occurrence　in　wheat　in　China　in　order　to　protect　consumers＇　health　from　the　toxins　exposure　risks.