Aiming　at　the　problems　of　low　secret　key　rate,　short　transmission　distance　and　low　detection　efficiency　of　continuous-variable　quantum　key　distribution　protocol,　we　propose　a　measurementdevice-independent　continuous-variable　quantum　key　distribution(MDI-CVQKD)　protocol　based　on　photon　subtraction　and　noiseless　linear　amplifiers　(NLA).　The　protocol　uses　non-Gaussian　operation,　in　particular,　photon　subtraction　to　improve　the　entanglement　between　quantum　states,　and　NLA　is　used　to　amplify　quantum　states　to　compensate　for　channel　attenuation.　In　this　paper,　the　principle　of　the　protocol　is　analyzed　theoretically,　and　the　relationship　between　the　secret　key　rate　and　the　secure　transmission　distance　is　derived　through　the　covariance　matrix　between　quantum　states.　Simulation　results　show　that　the　proposed　protocol　can　not　only　improve　the　entanglement　between　quantum　states,　but　also　extend　the　transmission　distance　of　key　distribution　and　improve　the　performance　of　key　distribution　system.