An　effective　and　precise　electrochemiluminescence　resonance　energy　transfer　(ECL-RET),　including　the　efficient　regulation　over　the　proximity　of　a　donor　and　an　acceptor　and　the　reliable　stimuli　responsive　as　well　as　the　avoidance　of　undesirable　probes　leakage,　etc.,　is　significant　for　the　development　of　an　accurate　and　sensitive　ECL　detection　method;　yet,　the　current　literature　in　documentation　involves　only　a　limited　range　of　such　ECL-RET　systems.　Herein,　we　propose　an　ECL-RET　strategy　with　dually　quenched　ultralow　background　signals　and　a　dual-stimuli　responsive,　accurate　signal　output　for　the　ultrasensitive　and　reliable　detection　of　anatoxin-a　(ATX-a).　The　dual　quenching　is　accomplished　by　an　integrated　ECL-RET　probe　of　metal　organic　frameworks　(MOFs)　encapsulated　into　Ru(bpy)(3)(2+)　(Ru-MOF)　(donor)　coated　with　silver　nanoparticles　(AgNPs)　shell　(acceptor　1)　and　close　proximity　with　DNA-ferrocene　(Fc)　(acceptor　2).　Multistimuli　responsive　DNAzyme　facilitated　the　accurate　signal　switch　by　both　target　ATX-a　and　hydrogen　peroxide　(H2O2).　Because　of　the　specific　recognition　of　the　aptamer　toward　ATX-a,　an　intricate　design　of　the　DNA　sequence　enabled　the　exposure　of　the　Ag+-dependent　DNAzyme　sequence　and　H2O2　in　situ　generated　Ag+　triggering　a　catalytic　cleavage　reaction　to　freely　release　the　two　ECL-RET　energy　acceptors,　thus　switching　the　ECL　signal　significantly　and　achieving　ultrasensitive　detection.　It　is　noteworthy　that　AgNPs　are　key　in　this　ECL-RET　strategy,　serving　both　as　the　gate-keepers　for　avoiding　ECL　probes　leakage　and　also　the　ECL　energy　acceptors,　and　mostly　importantly　serving　as　the　redox　substrate　for　the　subsequent　DNAzyme　catalytic　signal　switch.　The　proposed　ECL-RET　aptasensor　for　ATX-a　detection　displayed　splendid　monitoring　performance　with　a　quite　low　detection　limit　of　0.00034　mg　mL(-1).　This　sensor　not　only　led　to　the　development　of　a　dual-quenching　ECL-RET　system　but　also　provided　meaningful　multistimuli　responsive　ECL　biosensing　platform　construction,　which　shows　a　promising　application　prospect　in　complicated　sample　analysis.