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作者:

Shen, Feng (Shen, Feng.) | Li, Zonghe (Li, Zonghe.) | Ai, Mingzhu (Ai, Mingzhu.) | Gao, Hongkai (Gao, Hongkai.) | Liu, Zhaomiao (Liu, Zhaomiao.) (学者:刘赵淼)

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摘要:

The sorting of target particles from heterogeneous samples is challenging yet crucial for cell biology research and clinical diagnosis. Among various microfluidic methods, the use of cavity-based laminar vortex combined with inertial focusing is a powerful label-free passive technique for the selective sorting of large rare cells with high purity and concentration from billions of blood cells. However, this technology faces the challenge of improving the cavity holding capacity of trapped particles. This paper describes a round cavity-based vortex sorting method and presents a novel judgment criterion. The proposed round cavity achieves a holding capacity of entrapped target particles that is 2.2-7.8 times higher than that of rectangular cavities. By comparing the particle recirculating orbits and the simulated vortex morphology in round and rectangular cavities, a mechanism whereby particles/cells are held within the cavities is investigated. It is found that the area ratios (S = Ap/Ac) of the particle orbit area (Ap) to the cavity area (Ac) are 0.56 and 0.95 for the rectangular and round cavities, respectively. The results show that the round cavity provides more efficient space for recirculating particles and has better sorting performance. This round cavity-based vortex sorting method will be useful for clinical applications. Published under an exclusive license by AIP Publishing.

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作者机构:

  • [ 1 ] [Shen, Feng]Beijing Univ Technol, Fac Mat & Mfg, Beijing 100124, Peoples R China
  • [ 2 ] [Li, Zonghe]Beijing Univ Technol, Fac Mat & Mfg, Beijing 100124, Peoples R China
  • [ 3 ] [Ai, Mingzhu]Beijing Univ Technol, Fac Mat & Mfg, Beijing 100124, Peoples R China
  • [ 4 ] [Liu, Zhaomiao]Beijing Univ Technol, Fac Mat & Mfg, Beijing 100124, Peoples R China
  • [ 5 ] [Shen, Feng]Beijing Univ Technol, Beijing Key Lab Adv Mfg Technol, Beijing, Peoples R China
  • [ 6 ] [Liu, Zhaomiao]Beijing Univ Technol, Beijing Key Lab Adv Mfg Technol, Beijing, Peoples R China
  • [ 7 ] [Gao, Hongkai]Chinese Peoples Liberat Army Gen Hosp, Dept Gen Surg, Med Ctr 1, Beijing 100853, Peoples R China

通讯作者信息:

  • 刘赵淼

    [Liu, Zhaomiao]Beijing Univ Technol, Fac Mat & Mfg, Beijing 100124, Peoples R China;;[Liu, Zhaomiao]Beijing Univ Technol, Beijing Key Lab Adv Mfg Technol, Beijing, Peoples R China

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来源 :

PHYSICS OF FLUIDS

ISSN: 1070-6631

年份: 2021

期: 8

卷: 33

4 . 6 0 0

JCR@2022

ESI学科: PHYSICS;

ESI高被引阀值:7

被引次数:

WoS核心集被引频次: 8

SCOPUS被引频次: 9

ESI高被引论文在榜: 0 展开所有

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中文被引频次:

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