Wood　truss　joist　floors　are　increasingly　used　to　replace　traditional　solid　timber　joist　floors　in　low-rise　timber　houses.　An　understanding　of　the　vibration　performance　of　wood　truss　joist　floors　is　critical　for　the　design　and　serviceability　of　the　floors.　It　is　difficult　to　model　wood　truss　joist　floors　accurately　because　of　the　complicated　boundary　conditions　and　numerous　sophisticated　flexible　connections.　This　paper　discusses　three　simplified　modeling　methods　for　the　wood　truss　joist　floor　system.　The　modeling　results　were　validated　by　a　series　of　static　deflection　tests　and　vibration　modes　and　frequencies　tests　of　a　full-size　floor.　And　predictive　analysis　of　human-induced　vibration　of　the　floor　was　also　conducted.　The　vibration　characteristics　of　the　wood　truss　joist　floor　were　investigated.　The　examination　of　the　applicability　of　these　modeling　methods　was　provided.　The　results　indicate　that　the　point　loading　deflection　more　easily　affects　the　deflection　of　the　adjacent　joist.　However,　the　deflection　influence　on　other　joists　that　are　three　spaces　away　is　minimal.　Walking　on　the　wood　truss　joist　floor　produces　steep　vibration　acceleration　fluctuations　at　the　floor　center　for　a　relatively　long　time　period.　The　sheathing-to-joist　connections　and　the　metal　plate　connections　of　the　joists　have　significant　influences　on　the　vibration　response　of　the　wood　truss　joist　floor.　The　modeling　method,　which　considers　the　flexible　metal　plate　connections　and　flexible　sheathing-to-joist　connections,　performs　best　for　predicting　the　vibration　performance　of　the　floor.