• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
搜索

Author:

Han, Ying (Han, Ying.) | Qiu, Cuicui (Qiu, Cuicui.) | Li, Jiaojiao (Li, Jiaojiao.) | Gao, Fuping (Gao, Fuping.) | Yuan, Qing (Yuan, Qing.) | Tang, Yuhua (Tang, Yuhua.) | Niu, Wenchao (Niu, Wenchao.) | Wang, Xiayan (Wang, Xiayan.) | Gao, Xueyun (Gao, Xueyun.) | Gao, Liang (Gao, Liang.)

Indexed by:

EI Scopus SCIE PubMed

Abstract:

N-cadherin serves as an important oncobiomarker of epithelial-to-mesenchymal transition (EMT) progression, which identifies invasion and metastasis of malignant tumor cells. Although many efforts have been devoted to quantitative detection of N-cadherin, efforts to analyzing the protein of interest at intact cellular levels are scarce. Herein, a metal cluster-based electrochemical biosensing system is developed to determine the expressing levels of N-cadherin during the EMT process of tumor cells. To be specific, a peptide with a unique sequence and function is designed as a reductant and an anchor to synthesize metal clusters in a precise manner. Consequently, peptide-modified metal clusters possess N-cadherin-targeting, photoluminescence, and electrocatalytic properties. Especially, the redox-active metal clusters function as both an electron-transfer mediator and an electronic conductor for enhanced electrochemical sensing. These favorable features enable them as a rapid, sensitive, and reliable whole-cell biosensor, which integrates the fluorescence and electrochemical signals. This cytosensor can accurately quantify the expression levels of N-cadherin on at least 5000 tumor cells. Further, the current signals of model cancer cells gradually increase with EMT progression, indicating tumor cell-type evolution. Our study represents the advanced bioprobe and analytical methods for accurate quantitation of a biomarker to identify tumor progression.

Keyword:

in situ electrochemical analysis epithelial-to-mesenchymal transition N-cadherin metal clusters biosensor

Author Community:

  • [ 1 ] [Han, Ying]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 2 ] [Li, Jiaojiao]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 3 ] [Yuan, Qing]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 4 ] [Tang, Yuhua]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 5 ] [Niu, Wenchao]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 6 ] [Wang, Xiayan]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 7 ] [Gao, Xueyun]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 8 ] [Gao, Liang]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 9 ] [Qiu, Cuicui]Tsinghua Univ, State Key Lab Tribol, Beijing 100084, Peoples R China
  • [ 10 ] [Gao, Fuping]Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China

Reprint Author's Address:

  • [Gao, Liang]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China

Show more details

Related Keywords:

Source :

ACS SENSORS

ISSN: 2379-3694

Year: 2021

Issue: 6

Volume: 6

Page: 2290-2298

8 . 9 0 0

JCR@2022

JCR Journal Grade:1

Cited Count:

WoS CC Cited Count: 13

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

Affiliated Colleges:

Online/Total:627/5544297
Address:BJUT Library(100 Pingleyuan,Chaoyang District,Beijing 100124, China Post Code:100124) Contact Us:010-67392185
Copyright:BJUT Library Technical Support:Beijing Aegean Software Co., Ltd.