Suitability　of　different　substrates　for　enriched　mixed　microbial　cultures　(MMCs)　is　of　importance　to　the　polyhydroxyalkanoate　(PHA)　fermentation　using　renewable　carbon.　In　this　study,　three　enriched　MMCs　were　evaluated　for　their　fermentation　features　and　kinetics　with　different　carbon　sources　(sodium　acetate,　glucose,　or　starch).　The　results　showed　that　the　highly　specific　bacterial　community　composition　was　developed　depending　on　the　applied　carbon　source.　Correspondence　analysis　suggested　that　the　genus　affiliated　in　Gammaproteobacteria_unclassified　was　related　to　3-hydroxybutyrate　(HB)　synthesis　in　acetate-fed　MMC　(relative　abundance　of　38%)　and　glucose-fed　MMC　(relative　abundance　of　76.7%),　whereas　Vibrio　genus　was　related　to　3-hydroxyvalerate　(HV)　production　in　glucose-fed　MMC　(relative　abundance　of　0.4%)　and　starch-fed　MMC　(relative　abundance　of　94.6%).　The　acetate-fed　MMC　could　not　use　glucose　and　starch　as　fermentation　carbon　sources,　showing　the　limitation　of　microbial　species　developed　with　the　specific　metabolic　substrate.　Glucose-fed　MMC　produced　the　highest　PHA　cell　content　of　64.2%　cell　dry　weight　when　using　sodium　acetate　as　the　fermentation　carbon.　Glucose-fed　MMC　showed　wide　resilience　and　adaptation　to　various　carbon　sources.　When　actual　landfill　leachate　was　used　for　fermentation　by　glucose-fed　MMC,　maximum　PHA　cell　content　of　45.5%　cell　dry　weight　and　the　PHA　volumetric　productivity　of　0.265　g　PHA/(L　center　dot　h)　were　obtained.　This　study　suggested　carbon　sources　applied　in　the　MMC　enrichment　stage　had　a　significant　influence　on　utilization　of　carbon　in　the　fermentation　stage.