To　solve　the　problems　of　a　slow　start,　easy　loss,　and　easily　inhibited　activity　of　the　Anammox　bacteria　suspension　culture,　polyvinyl　alcohol-polypropylene　(PVA-PP)　was　used　to　prepare　the　Anammox　immobilized　filler.　To　improve　the　activity　of　Anammox　bacteria　and　ensure　stable　operation　of　the　reaction　system,　the　effects　of　COD　interference,　change　in　pH　value,　and　rotating　speed　on　the　nitrogen　removal　characteristics　of　the　immobilized　filler　were　determined　in　batch　tests.　Changes　in　the　structure　and　diversity　of　the　bacteria　in　the　filler　were　analyzed　by　a　high-throughput　sequencing　technique.　The　results　showed　that　the　activity　of　Anammox　bacteria　could　recover　to　100%　on　the　30th　day,　and　the　total　nitrogen　removal　rate　was　87.7%　when　the　total　nitrogen　volume　load　(NLR)　was　0.69　kg•(m3•d)-1　at　the　stage　of　99　days.　After　140　days　of　long-term　operation,　the　total　nitrogen　removal　rate　(NRR)　reached　1.83　kg•(m3•d)-1,　which　was　9.4　times　the　suspended　sludge　before　immobilization.　The　diversity　of　the　population　was　maintained　in　the　inclusion　carrier,　and　the　effective　enrichment　of　Candidatus　Kuenenia　(AF375995.1),　which　performs　anaerobic　ammonia-oxidization,　increased　from　11.06%　to　32.55%.　The　influence　of　COD　interference　and　changes　in　the　pH　value　of　Anammox　bacteria　was　significantly　weakened,　and　the　PVA-PP　entrapped　carrier　could　achieve　the　coupling　removal　of　nitrogen　by　Anammox　and　denitrification.　Appropriate　external　hydraulic　disturbance　would　promote　the　Anammox　reaction　in　immobilized　systems.　©　2020,　Science　Press.　All　right　reserved.