In　order　to　simulate　the　heat　transfer　in　the　process　of　hyperthermia,　one-dimensional　time-fractional　Cattaneo　heat　transfer　equation　(TFHE)　is　established.　Based　on　TFHE,　the　heat　transfer　model　is　solved　by　using　finite　difference　method,　because　a　single　layer　of　biological　tissue　in　vitro　is　irradiated　by　electromagnetic　energy.　The　effect　of　power　parameters　(energy　flux　density　P-0,　tissue　attenuation　coefficient　h)　and　equation　parameters　(relaxation　time　tau(q)　and　fractional　order　beta)　on　the　prediction　of　temperature　simulated　by　TFHE　were　studied.　Furthermore,　comparative　studies　on　TFHE,　Pennes　and　CV　are　performed　and　evaluated.　In　the　heating　process,　because　of　the　existence　of　relaxation　time　tau(q),　the　temperature　response　of　TFHE　and　CV　are　later　than　Pennes,　leading　to　the　lower　temperature　prediction　of　TFHE　and　CV　than　that　of　Pennes.　The　shorter　the　time　is,　the　higher　the　energy　is,　and　the　more　obvious　the　difference　is.