Hawthorn　(Crataegus　pinnatifida　Bunge)　is　a　common　agricultural　product　with　applications　in　medicine　and　food　in　China.　In　particular,　the　flavonoids　in　hawthorn　have　important　medicinal　effects.　To　quantify　the　flavonoid　contents　in　hawthorn　fruit　of　different　regions　in　China　and　scientifically　guide　the　reasonable　consumption　of　hawthorn,　12　flavonoids　in　hawthorn　samples　from　Shandong　(SD),　Henan　(HN),　Shaanxi　(SAX),　Shanxi　(SX),　and　Jiangsu　(JS)　were　detected　by　means　of　high　performance　liquid　chromatography　(HPLC).　The　total　flavonoid　content　in　the　Henan　sample　from　hawthorn　was　the　highest,　at　177.94　mu　g.g(-1),　and　the　Jiangsu　sample　was　the　lowest　at　124.67　mu　g.g(-1).　Epicatechin　was　the　flavonoid　with　the　highest　concentration　in　samples　from　all　regions,　and　the　average　content　in　Shandong　samples　reached　73.44　mu　g.g(-1).　Principal　component　analysis　(PCA),　hierarchical　cluster　analysis　(HCA)　and　discriminant　analysis　(DA)　were　also　performed　to　evaluate　the　effect　of　different　growing　regions　in　China　on　the　contents　of　flavonoids　in　hawthorn.　We　found　the　Jiangsu　samples　had　obvious　regional　characteristics,　and　the　compositions　of　(+/-)-catechin　hydrate,　epicatechin　and　phlorizin　dihydrate　could　be　used　to　distinguish　hawthorn　grown　in　Jiangsu　from　the　other　four　producing　areas.　The　results　will　provide　the　reference　basis　for　Chinese　hawthorn　consumers.