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Illumination properties in imaging system have profound influence on the imaging characteristics that is ultimately observed. Structured illumination has been applied widely to three-dimensional microscopic imaging for obtaining super-resolution and sectional imaging. It is obvious that optimized and adaptive structured illumination results in expected image. Three-dimensional structured illumination can be produced by interference of multi-beam which is diffracted from a certain grating. Focusing on structured-light formed by multi-beam interference,the effect of phase grating modulation parameters on the properties of structured-light pattern are investigated in this work. Both simulated and experimental results show that the phase grating has higher diffraction efficiency and the phase modulation parameter of the sinusoidal phase grating has direct impact on the distribution of the structured light pattern. When the phase modulation parameter is optimized, the maximum modulation of structured-light pattern in three-dimension is obtained. Then we simulated the imaging of Fresnel incoherent correlation holography with optimized 3D structured illumination, and presented the improved reconstruction images.
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