The　use　of　an　embedded　broad-spectrum　high-efficiency　denitrification　filler　to　treat　secondary　effluent　from　municipal　wastewater　treatment　plants　can　effectively　reduce　total　nitrogen　(TN)　concentration　of　the　effluent.　This　study　consists　of　two　parts.　The　D1　stage　studies　the　adaptability　of　the　secondary　effluent　based　on　the　embedded　denitrification,　removal　effect　of　total　nitrogen,　stable　working　conditions,　and　backwashing　conditions;　In　the　D2　stage,　the　change　in　the　nitrogen　removal　performance　of　the　filler　under　the　condition　of　a　year-long　stable　operation　was　studied.　The　variation　in　the　microbial　population　before　and　after　the　operation　of　the　embedded　packing　was　studied　by　high-throughput　sequencing　and　real-time　quantitative　PCR　detecting　system　real-time　(qPCR).　In　this　research,　the　embedded　denitrification　filler　had　a　water　temperature　of　(24±1),　pH:　7.1,　hydraulic　retention　time　(HRT):　1　h,　and　filling　rate:　10%.　Sodium　acetate　was　added　to　ensure　stable　operation　for　seven　days.　Under　adequate　carbon　source　conditions,　the　filler　can　adapt　to　the　quality　of　secondary　effluent　water　and　achieve　effluent　TN-1.　By　comparing　and　studying　the　effect　of　different　HRT　on　the　removal　of　filler　TN,　it　is　concluded　that　HRT　is　30　min　and　the　filling　rate　is　10%.　After　a　year　of　stable　operation　under　7.2　m3•d-1influent　conditions,　the　TN　removal　rate　can　reach　90.42%,　and　the　total　nitrogen　in　the　effluent　can　be　stabilized　below　5　mg•L-1.　In　comparison　with　the　backwashing　effect,　the　backwashing　strength　was　5.2　L•(m2•s)-1,　and　the　cycle　is　three　days　long.　High-throughput　sequencing　and　real-time　quantitative　PCR　analysis　results　show　that　the　abundance　and　copy　number　of　denitrifying　functional　genus　in　the　filler　before　and　after　the　operation　exhibited　significant　changes,　which　indicated　that　the　bacteria　could　achieve　good　self-growth　under　embedding　conditions.　©　2020,　Science　Press.　All　right　reserved.