Anammox　is　sensitive　to　temperature,　which　can　limit　its　practical　application　in　wastewater　treatment.　In　this　study,　a　step-feed　anoxic-oxic　(A/O)　process　coupled　with　PD/A　was　operated　steadily　from　26.8　degrees　C　to　13.1　degrees　C　for　wastewater　treatment　for　200　days.　The　effluent　total　inorganic　nitrogen　(TIN)　and　phosphorus　concentrations　were　10.2　mg/L　and　0.29　mg/L　at　C/N　ratio　of　4.6　and　15.0　degrees　C　even　with　increasing　nitrogen　loading　rate　(NLR).　The　anammox　activity　was　5.60　mg　NH4+-N/gMLSS/d　even　at　14　degrees　C,　moreover,　anammox　abundance　on　the　biocarriers　increased　with　decreasing　temperature.　It　was　observed　that　the　effect　of　partial　denitrification　(PD)　was　enhanced　under　low　temperature,　thus　the　contribution　of　anammox　for　nitrogen　removal　was　improved.　The　pathway　of　anammox　for　nitrogen　removal　accounted　for　48%　and　the　effect　of　effluent　did　not　deteriorate　under　low　temperature.　This　study　states　that　PD/A　has　advantages　under　low　temperature　operation,　which　is　suitable　for　treatment　of　wastewater　with　low　C/N　ratio.　(C)　2021　Elsevier　B.V.　All　rights　reserved.