This　paper　proposes　a　new　method　for　seismic　fragility　analysis　of　nuclear　power　plants　(NPPs)　considering　the　uncertainty　sources　of　both　ground　motions　and　structural　parameters.　Based　on　the　conventional　seismic　fragility　model　that　only　contains　the　uncertainty　of　ground　motions,　seismic　fragility　analysis　conditioned　on　a　specific　seismic　intensity　is　probabilistically　reformulated　by　further　incorporating　the　uncertainty　of　structural　parameters.　To　improve　the　efficiency　of　the　analysis,　a　point　estimation-assisted　incremental　dynamic　analysis　was　developed　for　evaluating　the　seismic　fragility　model　including　the　uncertainty　of　structural　parameter.　The　accuracy　and　efficiency　of　the　proposed　method　were　demonstrated　by　a　single-degree-of-freedom　system,　where　Monte　Carlo　simulation　method　is　used　for　comparison.　The　seismic　fragility　based　on　the　finite　element　model　of　an　AP1000　NPP　was　analyzed　using　the　proposed　method.　The　results　reveal　that　the　safety　of　NPP　structure　would　be　overestimated　without　considering　the　uncertainty　of　structural　parameter　and　the　influence　of　the　uncertainty　of　structural　parameters　on　the　seismic　fragility　of　NPP　presents　an　upward　trend　as　the　damage　level　increases.　Furthermore,　the　sensitivity　analysis　of　different　structural　parameters　reveals　that　elastic　module　may　have　a　dominant　influence.