The　association　between　the　footwear　microclimate　and　microbial　community　on　the　foot　plantar　skin　was　investigated　by　experiments　with　three　participants.　Novel　methods　were　developed　for　measuring　in-shoe　temperature　and　humidity　at　five　footwear　regions,　as　well　as　the　overall　ventilation　rate　inside　the　footwear.　Three　types　of　footwear　were　tested　including　casual　shoes,　running　shoes,　and　perforated　shoes　for　pairwise　comparison　of　footwear　microclimate　and　corresponding　microbial　community　on　the　skin.　The　major　findings　are　as　follows:　(1)　footwear　types　make　a　significant　difference　to　in-shoe　temperature　at　the　instep　region　with　the　casual　shoes　sustaining　the　warmest　of　all　types;　(2)　significant　differences　were　observed　in　local　internal　absolute　humidity　between　footwear　types,　with　the　casual　shoes　sustaining　the　highest　level　of　humidity　at　most　regions;　(3)　the　perforated　shoes　provided　the　highest　ventilation　rate,　followed　by　running　and　casual　shoes,　and　the　faster　the　gait,　the　larger　the　discrepancy　in　ventilation　rate　between　footwear　types;　(4)　the　casual　shoes　seemed　to　provide　the　most　favorable　internal　environment　for　bacterial　growth　at　the　distal　plantar　skin;　and　(5)　the　bacterial　growth　at　the　distal　plantar　skin　showed　a　positive　linear　correlation　with　the　in-shoe　temperature　and　absolute　humidity,　and　a　negative　linear　correlation　with　the　ventilation　rate.　The　ventilation　rate　seemed　to　be　a　more　reliable　indicator　of　the　bacterial　growth.　Above　all,　we　can　conclude　that　footwear　microclimate　varies　in　footwear　types,　which　makes　contributions　to　the　bacterial　growth　on　the　foot　plantar　skin.