For　comprehensive　insights　into　the　effects　of　disinfection　strategy　on　antibiotic　resistance　genes　(ARGs)　in　pipe　wall　biofilm　to　ensure　the　biosafety　of　domestic　hot　water　system　(DHWS),　this　study　investigated　the　occurrence,　removal　and　interactions　of　ARGs,　as　well　as　their　potential　hosts.　The　shifts　in　biofilm　community　composition　and　microbial　functions　(level　three)　were　observed　under　different　disinfection　strategies.　A　total　of　40　ARG　subtypes　within　seven　antibiotic　types　were　predicted.　The　total　gene　counts　ranged　from　6.10　x　104　to　2.31　x　105,　and　decreased　after　the　chlorine,　chlorine　dioxide,　silver　ions　(Ag+),　ultraviolet　(UV),　and　Ag+-UV　disinfection.　The　ARGs　in　samples　under　different　disinfection　strategies　were　divided　into　four　groups,　and　the　removal　and　enrichment　of　the　same　ARGs　in　each　group　were　almost　consistent.　The　complex　interactions　among　ARGs　were　weakened　and　altered　by　disinfection　(edges　decreased　from　237　to　178),　with　yebQ　and　marC　as　the　hub　ARGs,　but　positive　correlations　were　still　dominant.　Moreover,　36　nonpathogenic　bacteria,　including　high-　(Blastocatella),　mid-　(Ornatilinea)　and　low-abundant　genera　(Bdellovibrio),　as　well　as　18　potential　pathogens　(Vibrio　and　Bacillus)　were　identified　as　the　potential　hosts　harboring　ARGs.　Overall,　this　study　provides　some　new　insights　into　the　occurrence　of　ARGs　and　their　fate　under　eight　disinfection　strategies,　which　is　of　great　significance　for　a　comprehensive　understanding　and　control　of　ARGs　in　DHWS.