The　separation　of　molybdenum　(Mo)　from　tungstate　solution　is　a　bottleneck　problem　in　tungsten　(W)　metallurgy,　and　it　hinders　the　development　of　high-purity　tungsten　materials.　In　this　research,　a　modified　D301　resin　was　used　to　adsorb　and　separate　molybdenum　from　tungstate　solution.　The　maximum　sorption　capacity　(Q(e))　of　modified　D301　for　MoS42-　was　found　to　be　428　mg　g(-1)　and　the　separation　coefficient　(beta)　was　108.9　when　the　contact　time　was　4　h　and　the　reaction　temperature　was　25　degrees　C　and　the　pH　value　of　the　tungstate　solution　was　7.2.　The　sorption　process　conforms　to　Langmuir　isotherm　models　and　the　quasi-second-order　kinetic　model.　The　sorption　mechanism　was　also　discussed,　which　was　a　single　layered　spontaneous　sorption　process.　Theoretical　calculations　infer　bonding　behavior　between　the　N　atom　on　the　resin　and　the　S　atom　on　the　MoS42-　molecule.　The　sorption　energy　is　-7.67　eV,　which　indicated　that　the　sorption　process　is　stable　chemical　sorption.　The　desorption　experiment　showed　that　more　than　90%　molybdenum　could　be　desorbed　from　the　loaded　resin　when　the　concentration　of　sodium　hydroxide　solution　was　5　w%.　Finally,　after　three-stage　sorption-desorption,　almost　all　molybdenum　in　the　solution　was　adsorbed,　achieving　better　separation　of　tungsten　and　molybdenum.