The　plastic　deformation　behavior　of　three　various　orientation　specimens　of　a　nickel-based　single-crystal　(SX)　superalloy　were　investigated　by　in-situ　electron　back-scattered　diffraction　(EBSD)　at　room　temperature.　Tensile　properties,　slip　morphology,　the　evolution　of　Schmid　factor,　lattice　rotation,　and　local　strain　were　thoroughly　discussed.　In　[1　1　13]　specimen,　the　interaction　of　multi　slip　behavior　induced　local　strain　concentration,　which　restricted　the　plasticity　but　enhanced　the　strain　hardening.　Due　to　the　single　slip　behavior,　lower　tensile　strength　and　higher　elongation　were　discovered　in　[9　1　7]　and　[2　1　3]　specimens.　Furthermore,　during　the　plastic　deformation　of　[1　1　13]　specimen,　lattice　rotation　is　confined　around　[0　0　1]　pole　and　shows　a　dispersive　distribution,　which　is　triggered　by　the　multi　slip　behavior.　A　modified　Sachs　model　considering　multi　slip　behavior　has　been　developed　to　predict　the　lattice　rotation,　this　model　is　also　applicable　to　severely　deformed　grains　in　polycrystalline　materials.