This　study　aims　to　develop　a　simple,　sensitive,　low-cost,　environmentally　friendly　and　flexible　surface-enhanced　Raman　scattering　(SERS)　platform,　combined　with　a　portable　Raman　spectrometer,　for　the　rapid　and　on-site　SERS　detection　of　bacteria.　Commercial　tobacco　packaging　paper　(TPP)　with　little　background　interference　was　used　as　a　loading　medium　that　effectively　adsorbed　Au　nanoparticles　and　provided　sufficient　＂hot　spots＂.　This　Au-tobacco　packaging　paper　(Au-TPP)　substrate　used　as　a　flexible　SERS　platform　can　maximize　sample　collection　by　wiping　irregular　surfaces,　and　was　successfully　applied　to　the　on-site　and　rapid　detection　of　pathogenic　bacteria.　Raman　fingerprints　of　pathogenic　bacteria　can　be　obtained　by　SERS　detection　of　spiked　pork　using　wipeable　Au-TPP,　which　verifies　its　value　in　practical　applications.　The　results　collected　by　SERS　were　further　verified　by　polymerase　chain　reaction　(PCR)　results.　It　showed　several　advantages　in　on-site　SERS　detection,　including　accurate　discrimination,　simple　preparation,　easy　operation,　good　sensitivity,　accuracy　and　reproducibility.　This　study　indicates　that　the　established　flexible　SERS　platform　has　good　practical　applications　in　pathogenic　bacterial　identification　and　other　rapid　detections.