Antibiotic　resistance　genes　(ARGs)　and　antibiotic　resistant　bacteria　(ARB)　are　widespread　in　urban　wastewater　treatment　plants　(UWTPs).　In　this　research,　a　horizontal　transfer　model　of　recipient　(Pseudomonas.　HLS-6)　and　donor　(Escherichia　coli　DH5　alpha　carries　RP4　plasmid)　was　constructed　to　explore　the　effect　of　sulfidated　nanoscale　zerovalent　iron　(S-nZVI)　on　the　efficiency　of　plasmid-mediated　horizontal　transfer.　When　the　S/Fe　was　0.1,　the　inactivation　efficiency　of　1120　mg/L　S-nZVI　on　the　donor　and　recipient　bacteria　were　2.36　+/-　0.03　log　and　3.50　+/-　0.17　log　after　30　min,　respectively　(initial　ARB　concentration　asymptotic　to　5　x10(7)　CFU/mL).　Effects　of　treatment　time,　S/Fe　molar　ratio,　S-nZVI　dosage　and　initial　bacterial　concentration　were　systemically　studied.　S-nZVI　treatment　could　increase　the　extracellular　alkaline　phosphatase　and　malondialdehyde　content　of　the　ARB,　cause　oxidative　stress　in　the　bacteria,　destroy　the　cell　structure　and　damage　the　intracellular　DNA.　This　study　provided　evidence　and　insights　into　possible　underlying　mechanisms　for　reducing　conjugative　transfer,　such　as　hindering　cell　membrane　repair,　inducing　the　overproduction　of　reactive　oxygen　species,　inhibiting　the　SOS　response,　reducing　the　expression　of　ARGs　and　related　transfer　genes.　S-nZVI　could　inhibit　the　gene　conjugative　transfer　while　inactivating　the　ARB.　The　findings　provided　an　alternative　method　for　controlling　antibiotic　resistance.