Anammox　process　has　been　widely　regarded　as　an　energy-efficient　method　for　sludge　digestion　filtrate　treatment.　However,　the　complex　high-strength　organics　in　the　filtrate,　especially　of　Anaerobic　Digestion　after　Thermal　Hydrolysis　Pretreatment　(THP-AD),　brings　serious　threat　to　anammox　bacteria,　and　the　high　nitrate　residue　in　effluent　remains　another　significant　barrier　in　operation.　In　this　study,　a　novel　double-line　anammox-mediated　system,　integrating　the　Partial　Nitrification/Anammox　(PNA)　with　Partial　Denitrification/Anammox　(PDA)　processes　in　separately　sequencing　batch　reactors　(SBRs),　was　developed　to　polish　the　THP-AD　filtrate.　When　the　real　THP-AD　filtrate　(1946.5　mg　NH4+-N/L,　2076.0　mg　COD/L)　was　fed　to　the　front　PNA　reactor　(SBRPNA)　with　5-fold　dilution,　effluent　total　nitrogen　(TN)　remained　at　93.0　mg/L.　Notably,　the　final　effluent　TN　was　effectively　polished　to　as　low　as　8.8　mg/L　by　the　following　PDA　reactor　(SBRPDA),　which　was　fed　with　the　SBRPNA　effluent　and　real　domestic　wastewater　(71.0　mg　NH4+-N/L,　209.1　mg　COD/L).　More　severe　inhibition　on　anammox　activity　was　observed　in　SBRPNA　rather　than　SBRPDA　by　refractory　organics　in　filtrate.　Fortunately,　it　could　be　alleviated　with　the　enhanced　degradability　of　particulate　organics　and　aromatic　protein-like　compounds,　attributed　to　the　enrichment　of　class　Anaerolineae　in　both　SBRPNA　and　SBRPDA.　This　further　stimulated　the　electron　donor　supply　for　PDA　process　with　much　lower　external　carbon　source　demand.　16S　rRNA　sequencing　analysis　revealed　that　Candidatus　Brocadia　as　dominant　anammox　bacteria　were　efficiently　enriched　in　both　SBRPNA　and　SBRPDA,　indicating　its　unexpected　toughness　and　adaptability　to　the　complex　organic　compounds　in　THP-AD　filtrate.　Overall,　this　study　suggested　that　the　novel　double-line　anammox　would　be　a　promising　alternative　for　cost-efficient　nitrogen　removal　from　high-strength　wastewater　containing　complex　organic　matter.