Diabetic　wounds　are　a　worldwide　health　problem,　with　increasing　morbidity　and　risk　of　amputation.　This　study　investigated　a　novel　application　of　a　human　decellularized　adipose　tissue　matrix　(hDAM)　as　a　natural　3D　scaffold　for　delivering　human　adipose-derived　stem　cells　(hASCs)　to　diabetic　wounds.　The　porous　structure,　ability　to　preserve　extracellular　matrix　components,　and　convenient　storage　conditions　of　decellularized　hDAM　make　it　a　potential　clinical　wound　dressing　material.　The　hASCs　cultured　in　the　hDAM　scaffold　exhibited　a　fibroblast-like　morphology　and　more　evenly　distributed　cells　on　both　the　surface　and　inside　the　porous　structure　of　the　hDAM　scaffold.　In　addition,　the　biocompatibility　of　hDAM　enhanced　the　hASCs　proliferation,　maintenance　of　the　stemness　properties,　and　release　of　angiogenic　cytokines　compared　to　those　under　standard　culture　conditions.　Moreover,　cell　suspensions　derived　from　hASCs　cultured　in　hDAM　scaffolds　promoted　the　proliferation　and　migration　of　human　umbilical　vascular　endothelial　cells,　indicating　its　potential　effect　in　promoting　angiogenesis.　Furthermore,　the　potential　clinical　therapeutic　efficacy　of　the　hASC-hDAM　composite　for　diabetic　wound　healing　was　evaluated　using　a　full-thickness　wound　model　in　diabetic　mice.　Diabetic　rats　treated　with　the　hASCsseeded　hDAM　scaffold　displayed　enhanced　wound　healing　efficiency,　including　an　improved　blood　perfusion　volume　for　wounds,　reduced　number　of　inflammatory　cells,　and　enhanced　epithelization.　This　study　demonstrated　that　the　3D　model　that　combined　hASCs　and　an　hDAM　could　accelerate　wound　healing　and　might　hold　potential　for　clinical　application　to　enhance　diabetic　wound　healing　and　regeneration.