A　hydrophobic　poly(tetrafluoroethylene)　(PTFE)　porous　membrane　was　modified　into　a　hydrophilic　lithium-ion　battery　(LIB)　separator　with　a　higher　affinity　to　the　electrolyte　via　the　hybrid　consisting　of　a　cationic　fluorocarbon　surfactant　(FCS),　polyethylenimine　(PEI),　and　tetraethyl　orthosilicate　(TEOS),　which　can　be　in-situ　biomineralized　into　SiO2　nanoparticles.　The　obtained　PTFE/FCS-PEI-SiO2　separator　showed　a　dynamic　electrolyte　contact　angle,　which　decreased　from　50.3　degrees　to　0　degrees　within　4　s,　along　with　215　%　of　electrolyte　uptake.　The　ionic　conductivity　and　the　interfacial　resistance　were　9.12　mS　cm(-1)　and　228.8　omega,　respectively.　In　addition,　the　cell　equipped　with　the　separator　showed　a　capacity　of　163.7　mAh　g(-1)　at　a　current　density　of　0.2　C.　Notably,　the　cell　with　the　separator　displayed　good　high-temperature　stability.