Tetrasphaera　are　polyphosphate　accumulating　organisms　(PAOs)　that　play　an　important　role　in　enhanced　biological　phosphorus　removal　(EBPR)　from　wastewater.　The　effect　of　a　wide　range　of　temperature　changes　(1-30　degrees　C)　on　phosphorus　removal,　metabolism　and　clade-level　community　structure　of　Tetrasphaera-dominated　PAOs　was　investigated.　At　10　degrees　C,　the　bioactivities　of　Tetrasphaera-dominated　communities　were　obviously　inhibited　and　the　EBPR　efficiency　was　only　73　%.　Yet　at　20-30　degrees　C,　EBPR　efficiency　reached　99　%　and　the　relative　abundance　of　Tetrasphaera　was　up　to　90　%.　The　temperature　variation　changed　the　community　distribution　of　Tetrasphaera　clades,　which　was　possibly　a　main　reason　for　EBPR　performance.　Amino　acids　and　PHA　with　different　contents　were　intracellular　metabolite　of　Tetrasphaera-dominated　communities　during　phosphorus　release　and　uptake　at　different　temperatures.　Moreover,　Tetrasphaera　fermented　protein　and　amino　acids　and　released　VFAs.　The　outcomes　suggested　the　great　potential　of　Tetrasphaera-PAOs　in　the　treatment　of　wastewater　with　varying　temperatures　and　limited　carbon　sources.