The　efficient　removal　of　nitrogen　and　phosphorus　remains　challenging　for　traditional　wastewater　treatment.　In　this　study,　the　feasibility　for　enhancing　the　partial-denitrification　and　anammox　process　by　Fe　(III)　reduction　coupled　to　anammox　and　nitrate-dependent　Fe　(II)　oxidation　was　explored　using　municipal　wastewater.　The　nitrogen　removal　efficiency　increased　from　75.5　%　to　83.0　%　by　adding　Fe　(III).　Batch　tests　showed　that　NH4+-N　was　first　oxidized　to　N-2　or　NO2--N　by　Fe　(III),　then　NO3--N　was　reduced　to　NO2--N　and　N(2　)by　Fe　(II),　and　finally,　NO2--N　was　utilized　by　anammox.　Furthermore,　the　performance　of　phosphorus　removal　improved　by　Fe　addition　and　the　removal　efficiency　increased　to　78.7　%.　High-throughput　sequencing　showed　that　the　Fe-reducing　bacteria　Pseudomonas　and　Thiobacillus　were　successfully　enriched.　The　abundance　of　anammox　bacterial　increased　from　0.03　%　to　0.22　%　by　multiple　nitrite　supply　pathways.　Fe　addition　presents　a　promising　pathway　for　application　in　the　anammox　process.