This　paper　systematically　investigated　the　roles　of　aging　and　bio-oil　regeneration　on　the　self-healing　evolution　behavior　of　pure　asphalt　and　terminal　blend　(TB)　rubberized　asphalt　within　wide　temperature　range,　which　was　expected　to　promote　the　applications　of　self-healing　asphalt　pavement,　meanwhile　to　accelerate　the　sustainable　disposal　of　local　bio-oil　and　rubber　wastes.　The　self-healing,　flow　and　elastic　recovery　(ER)　abilities　of　asphalts　within　wide　temperature　range　and　their　relationship　were　traced　through　the　dynamic　shear　rheometer　based　fatigue-healing-fatigue,　frequency　sweep　and　creep　recovery　tests.　Results　revealed　that　different　effects　of　temperature　on　ER　and　flow　abilities　of　aged　or　rejuvenated　asphalts　resulted　in　the　complexity　of　self-healing　evolution　behavior.　The　proposed　ER-flow　self-healing　theory　clarified　that　the　ER　ability　of　aged　asphalts　led　the　self-healing　process　in　viscoelastic　region,　and　the　flow　ability　dominated　as　temperature　rose　into　viscous　flow　region;　however,　the　flow　ability　of　regenerated　asphalts　dominated　the　self-healing　at　all　measured　temperatures.　The　aging　growth　percentage　and　regeneration　recovery　percentage　(%RR)　based　on　four　self-healing　characteristic　temperatures　were　proposed　to　quantify　the　influences　of　aging　and　regeneration　on　self-healing　evolution　behavior　of　asphalts.　The　climate　aging　imparted　severer　impact　on　the　self-healing　evolution　behavior　of　pure　asphalt　than　long-term　aging,　whereas　caused　less　impact　on　that　of　TB　asphalt.　The　aging　impact　on　the　self-healing　evolution　behavior　of　TB　asphalt　was　smaller　than　that　of　pure　asphalt.　Inversely,　the　%RR　of　rejuvenated　asphalts　increased　as　the　growth　of　bio-oil　content,　and　reached　the　maximum　when　adding　4%wt.　waste　cooking　oil　residues.　The　bio-oil　elicited　the　greater　regeneration　improvement　of　self-healing　performance　than　petroleum-based　rejuvenator.　Overall,　the　bio-oil　regeneration　recovery　of　the　self-healing　evolution　performance　of　aged　asphalts　was　limited.