Nanotopography　is　an　effective　method　to　regulate　cells＇　behaviors　to　improve　Ti　orthopaedic　implants＇　in　vivo　performance.　However,　the　mechanism　underlying　cellular　matrix-nanotopography　interactions　that　allows　the　modulation　of　cell　adhesion　has　remained　elusive.　In　this　study,　we　have　developed　novel　nanotopographic　features　on　Ti　substrates　and　studied　human　osteoblast　(HOb)　adhesion　on　nanotopographies　to　reveal　the　interactive　mechanism　regulating　cell　adhesion　and　spreading.　Through　nanoflat,　nanoconvex,　and　nanoconcave　TiO2　nanotopographies,　the　evolution　of　Coulomb＇s　force　between　the　extracellular　matrix　and　nanotopographies　has　been　estimated　and　comparatively　analyzed,　along　with　the　assessment　of　cellular　responses　of　HOb.　We　show　that　HObs　exhibited　greater　adhesion　and　spreading　on　nanoconvex　surfaces　where　they　formed　super　matured　focal　adhesions　and　an　ordered　actin　cytoskeleton.　It　also　demonstrated　that　Coulomb＇s　force　on　nanoconvex　features　exhibits　a　more　intense　and　concentrated　evolution　than　that　of　nanoconcave　features,　which　may　result　in　a　high　dense　distribution　of　fibronectin.　Thus,　this　work　is　meaningful　for　novel　Ti-based　orthopaedic　implants＇　surface　designs　for　enhancing　their　in　vivo　performance.