For　the　quantitative　analysis　of　medical　images　in　clinical　research,　diagnosis　and　treatment,　a　reliable　basic　framework　for　developing　a　normal　human　ear　atlas　of　voxel-based　computed　tomography　(CT)　images　was　proposed.　We　annotated　10　precise　ear　structures　with　different　labels　from　64　patients　with　normal　ear　structures.　Paired-samples　t　test,　Pearson’s　test　and　descriptive　statistics　were　carried　on　the　volume　and　coordinate　data,　which　were　first　obtained　from　annotation　to　verify　the　correlation　and　difference.　In　addition,　we　constructed　a　three　dimensional　(3D)　model　of　the　standard　human　ear　atlas　with　six　views　for　presentation.　Through　a　series　of　statistical　analyses,　a　standard　3D　normal　human　ear　atlas　containing　volume　and　spatial　data　was　obtained　from　voxel-based　CT　images.　There　was　a　significant　negative　correlation　exists　between　age　and　the　volume　of　the　incus,　and　no　correlation　with　other　structures.　There　was　no　significant　correlation　between　slice　thickness　and　the　volume　of　10　structures.　The　volume　of　most　structures　on　both　sides　is　significantly　correlated　and　there　was　no　significant　difference　in　the　volume　of　most　structures　on　both　sides　except　for　the　jugular　foramen.　Besides,　the　coordinate　range　of　the　bilateral　structures　is　relatively　consistent.　The　specific　volume　and　spatial　data　for　the　human　ear　atlas　are　helpful　in　the　diagnosis　of　abnormalities,　and　this　3D　normal　human　ear　atlas　will　provide　new　insights　for　radiologists　in　clinical　research.　©　2019,　Springer　Science+Business　Media,　LLC,　part　of　Springer　Nature.