The　bio-treatment　of　methanethiol　(MT)　and　dimethyl　sulfide　(DMS),　the　most　common　sulfur　compounds　in　odorous　gas,　is　difficult　due　to　the　inhibition　of　DMS　degradation　by　MT.　This　article　investigated　the　treatment　of　MT　and　DMS　odorous　gas　using　a　single-stage　biotrickling　filter　(BTF)　packed　with　polyurethane　foam　cubes　that　were　inoculated　with　activated　sludge　from　a　sewage　treatment　plant　operating　an　anaerobic/aerobic/oxic　(AAO)　process.　The　BTF　system　lasted　for　161　days　(with　9　days　to　startup)　under　an　empty　gas　residence　time　of　39　s.　The　elimination　capacities　for　MT　and　DMS　were　85.2　g/m(3)/h　(removal　efficiency　=　96.6%)　and　6.4　g/m(3)/h　(removal　efficiency　=　95.0%),　respectively,　and　the　maximal　elimination　capacities　of　MT　and　DMS　were　119.7　g/m(3)/h　and　7.3　g/m(3)/h,　respectively.　The　optimal　parameters　were　as　follows:　empty　bed　retention　time,　39　s;　pH,　6.1;　recirculation　medium　flow　rate,　＞=　1.2　m(3)/m(2)/h;　temperature,　29-36　degrees　C;　and　SO42-　concentration,　＜　2.0　g-SO42-/L.　Microbial　community　analysis　revealed　that　spatial　differentiation　between　MT-degrading　bacteria　and　DMS-degrading　bacteria　enable　the　single-stage　BTF　can　simultaneously　remove　MT　and　DMS.　The　activated　sludge　of　AAO　process　can　be　used　as　the　inoculation　sludge　to　treating　MT　and　DMS　gas,　which　provides　an　important　reference　for　the　industrial　application　of　treating　odorous　gas　containing　MT　and　DMS.　(C)　2019　Elsevier　Ltd.　All　rights　reserved.