To　accumulate　endogenous　polymers　during　the　aerobic　phase,　the　aerobic/anoxic-feast/famine　(O/A-F/F)　selection　mode　can　be　used.　It　can　also　be　used　in　situ　for　endogenous　denitrification　by　activated　sludge　during　the　anoxic　phase.　To　further　explore　the　effect　of　carbon　sources　on　the　activated　sludge　accumulation　of　endogenous　polymers　and　endogenous　denitrification,　this　study　used　acetic　and　glucose　as　the　main　carbon　sources　to　investigate　the　accumulation　of　endogenous　polymers,　endogenous　denitrification,　and　the　structure　and　function　of　enriched　activated　sludge.　The　results　show　that　acetic　(Ac-SBR)　and　glucose　(Gc-SBR)　as　the　main　carbon　source　systems　achieved　a　40　mg•L-1　nitrate　removal　by　endogenous　denitrification　when　the　influent　chemical　oxygen　demand　(COD)　was~500　mg•L-1　in　the　O/A-F/F　selection　mode.　Both　the　Ac-SBR　and　Gc-SBR　achieved　partial　denitrification,　but　the　nitrite　accumulation　of　the　Ac-SBR　was　higher　than　that　of　the　Gc-SBR.　Acetic　is　favorable　for　the　accumulation　of　endogenous　polyhydroxyalkanoate　(PHA);　PHA　drives　the　endogenous　denitrification.　The　yield　of　PHA　was　0.52　and　the　denitrification　rate　(DNR)　was　9.65　mg•(L•h)-1.　The　Gc-SBR　system　achieved　the　simultaneous　accumulation　of　PHA　and　glycogen　(Gly).　The　yield　of　Gly　was　higher　than　that　of　PHA　and　the　DNR　driven　by　Gly　was　4.35　mg•(L•h)-1.　The　Gly　was　the　main　driving　force　to　achieve　endogenous　denitrification　and　contributed　to　77%　of　the　total　nitrogen　removal.　The　16S　rRNA　high-throughput　sequencing　analysis　of　activated　sludge　flora　shows　that　the　class　of　β-Proteobacteria　in　the　Proteobacteria　was　dominant,　with　an　abundance　of　40.56%　in　the　Ac-SBR.　However,　the　abundance　of　β-Proteobacteria　was　only　18.05%　in　the　Gc-SBR.　The　class　of　α-Proteobacteria　contributes　to　glycogen　accumulation　in　the　Gc-SBR.　The　PHA　can　be　accumulated　by　β-Proteobacteria,　Unclassified　Bacteroidetes,　and　Lgnavibacteria　in　the　Ac-SBR.　©　2019,　Science　Press.　All　right　reserved.