Poor　operational　stability　and　effluent　manganese　(Mn)　concentration　exceeding　limitation　were　found　during　the　same　layer　of　purification　process　with　the　low-temperature　(5~6)　groundwater　containing　high　ammonia　nitrogen(NH3-N),　iron　(Fe)　and　Mn　contents　(TFe:9~15mg/L,　Fe2+:　6~12mg/L,　NH3-N:　1.4~2.0mg/L,　Mn2+:　1.4~2.0mg/L).　In　order　to　achieve　efficient　purification,　the　start-up　of　＇two-stage　aeration　combined　with　two-stage　filtration＇　purification　process　was　investigated　in　water　plant　purification　workshop,　and　the　two-stage　bio-purification　was　started　separately　by　using　the　differential　filtration　rate　and　the　same　filtration　rate.　Results　showed　that　two　startup　methods　were　started　successfully　after　59　days　and　106　days.　The　start-up　with　the　same　filter　speed　shortened　the　startup　time　effectively,　and　the　efficiency　of　Fe,　NH3-N　and　Mn　were　110.69g/(m2h),　18.80g/(m2h)　and　19.54g/(m2h)　respectively.　Fe　was　removed　to　trace　level　at　60cm　of　the　primary　filter　column;　NH3-N　was　evenly　removed　by　85.08%　in　the　primary　filter　column,　with　the　remaining　14.92%　removed　from　the　secondary　filter　column;　the　efficiency　and　the　oxidation-removal　active　sites　of　Mn　were　obviously　affected　by　the　filtration　rate　as　well　as　the　influent　NH3-N　concentration.　Mn　was　removed　by　33.72%　and　66.28%　in　the　primary　filtration　columnand　and　the　secondary　filtration　column,　respctively.　Mn　removal　remains　a　decisive　factor　in　filter　maturity.　©　2019,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.