For　the　rapid　cultivation　of　anaerobic　ammonium　oxidation　(ANAMMOX)　granular　sludge　(AGS),　a　small　amount　of　flocculent　ANAMMOX　sludge　(FAS)　was　taken　as　the　research　object,　and　a　bio-flow　separate　ball　was　used　as　the　filler　in　an　up-flow　anaerobic　sludge　bed　(UASB)　to　rapidly　begin　ANAMMOX　and　to　cultivate　granular　sludge.　In　addition,　the　substrate　inhibition　kinetic　characteristics　of　the　AGS　were　investigated　by　using　the　Haldane　model.　The　results　showed　that　start-up　of　the　ANAMMOX　was　successfully　achieved.　The　total　nitrogen　removal　rate　was　more　than　85%,　and　the　volume　load　of　total　nitrogen　was　0.72　kg•(m3•d)-1.　AGS　with　diameters　of　1.0-3.0　mm　were　cultured　within　127　days　using　the　Bio-flow　Separate　Ball.　The　kinetic　studies　showed　that　the　maximum　reaction　rates　for　ammonia　and　nitrite　of　granular　sludge　were　1.46　kg•(kg•d)-1　and　1.76　kg•(kg•d)-1,　with　half　inhibition　constants　of　ammonia　and　nitrite　at　852.2　mmol•L-1　and　108.2　mmol•L-1,　respectively.　Compared　with　FAS,　AGS　can　withstand　higher　concentrations　of　ammonia　and　nitrite　and　can　maintain　a　higher　reaction　rate.　The　placement　of　the　filler　has　positive　significance　for　starting　ANAMMOX　and　rapidly　culturing　AGS.　©　2019,　Science　Press.　All　right　reserved.