A　pilot-scale　bio-filter　was　constructed　for　the　removal　of　high　concentrations　of　iron　(TFe:　9~15mg/L,　Fe2+:　6~12mg/L),　manganese　(Mn2+:　0.8~1.2mg/L),　and　ammonia　nitrogen　(NH3-N:　0.9~1.4mg/L)　simultaneously　from　low　temperature　(5~6)　groundwater.　Pilot-scale　bio-filter　were　started　separately　by　using　the　1.0m/h　and　3.0m/h　were　started　successfully　after　128d　and　91d,　respectively.　Iron　and　ammonia　nitrogen　were　removed　at　the　beginning　of　the　bio-filter　start-up,　Mn　removal　remains　a　decisive　factor　in　filter　maturity.　The　required　thickness　of　the　bio-filter　required　for　purification　increased　as　the　concentration　of　ammonia　nitrogen　increased　when　dissolved　oxygen　(DO)　was　sufficient.　The　maximum　removal　of　ammonia　nitrogen　was　about　1.60mg/L.　DO　deficiency　was　the　main　factor　limiting　the　further　increase　of　ammonia　nitrogen.　For　higher　filtration　rates,　the　removal　of　manganese　was　less,　and　the　thicker　the　filter　layer　required　for　purification.　The　maximum　filtration　rate　of　the　single　bio-filter　was　8.0m/h.　Analysis　along　the　way,　there　were　significant　removal　classification　between　Fe　and　Mn　during　the　oxidation-removal　process,　iron　and　ammonia　nitrogen　in　the　filter　layer　can　be　oxidized　and　removed　simultaneously,　manganese　is　oxidized　and　removed　after　the　iron　and　ammonia　nitrogen.　©　2019,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.