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Using domestic wastewater as influent and carbon fibre as biological carriers, the characteristics of SND(simultaneous nitrification and denitrification) process and N2O emission were studied under different aeration intensity (30,20,10 L/h), with the DO(dissolved oxygen) concentration controlled at 1.5 mg/L by adjusting the ratio of N2 and O2. Hetertrophic and nitrifying bacteria conexisted in the biofilm, which are located in the outer and inner layers of the biofilm, respectively. The decrease of aeration intensity was beneficial to the proliferation of the external heterotrophic bacteria. With the aeration intensity was controlled at 30 L/h and 10 L/h, the removal efficiency of NH4+-N in the SBBR system were more than 95% and 79.2%±1.6%, the SND efficiency was 46.2%±2.6% and 62.1%±2.3%, the N2O yield was 6.25%±0.6% and 2.93%±0.43%, respectively. During the anoxic phase, the denitrification process and the accumulation of PHA (poly-β-hydroxyalkanoate) occurred simultaneously. In the following aerobic process, the PHA increased first and then decreased, which indicated that PHA could be used as internal carbon sources in the following denitrification process. Both the aerobic denitrification process of AOB and the denitrification process of denitrifying bacteria at the presence of lower oxygen led to the emissions of N2O. The decrease of aeration intensity leads to a decrease in hydraulic shearing force, an increase in the anoxic range in the biofilm, and an increase in the N2O residence time in the anoxic zone, which is beneficial to the denitrification reduction. As the aeration intensity decreased from 30 L/h to 10 L/h, the extracellular polymer (EPS) secretion decreased, and the PS/PN (polysaccharide/protein) decreased from 8.59 mg/mg to 6.58 mg/mg, resulting the reduction of the biofilm density, which created the favorable conditions for N2O transferring into the biofilm to complete the reduction process. © All Right Reserved.
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