Sulfide　and　methane　production　are　a　major　concern　in　sewer　management.　Many　solutions　with　the　use　of　chemicals　have　been　proposed　yet　incurring　huge　costs.　Here,　this　study　reports　an　alternative　solution　to　reduce　sulfide　and　methane　production　in　sewer　sediments.　This　is　achieved　through　integration　of　urine　source　sepa-ration,　rapid　storage,　and　intermittent　in　situ　re-dosing　into　a　sewer.　Based　on　a　reasonable　capacity　of　urine　collection,　an　intermittent　dosing　strategy　(i.e.　40　min　per　day)　was　designed　and　then　experimentally　tested　using　two　laboratory　sewer　sediment　reactors.　The　long-term　operation　showed　that　the　proposed　urine　dosing　in　the　experimental　reactor　effectively　reduced　sulfidogenic　and　methanogenic　activities　by　54%　and　83%,　compared　to　those　in　the　control　reactor.　In-sediment　chemical　and　microbial　analyses　revealed　that　the　short-term　exposure　to　urine　wastewater　was　effective　in　suppressing　sulfate-reducing　bacteria　and　methanogenic　archaea,　particularly　within　a　surface　active　zone　of　sediments　(0-0.5　cm)　likely　attributed　to　the　biocidal　effect　of　urine　free　ammonia.　Economic　and　environmental　assessments　indicated　that　the　proposed　urine　approach　can　save　91%　in　total　costs,　80%　in　energy　consumption　and　96%　in　greenhouse　gas　emissions　compared　to　the　conventional　use　of　chemicals　(including　ferric　salt,　nitrate,　sodium　hydroxide,　and　magnesium　hydroxide).　These　results　collectively　demonstrated　a　practical　solution　without　chemical　input　to　improve　sewer　management.