Sulfide-driven　partial　denitrification　and　anaerobic　ammonia　oxidizing　(anammox)　(SPDA)　is　a　high-efficiency　technology　to　achieve　simultaneous　nitrogen　and　sulfide　removal.　Nitrite　accumulation　from　sulfide-driven　partial　denitrification　is　the　key　to　achieve　SPDA.　Zeolite　powder　was　added　to　strengthen　the　competition　of　anammox　bacteria　against　nitrite.　The　nitrogen　removal　rate　(NRR)　and　partial　denitrification　efficiency　in　reactor　was　5.18　kg-N　m　�　3d-1　and　92.3%　during　180　days　of　operation,　higher　than　those　without　zeolite　powder,　indicating　an　improving　contribution　of　zeolite　powder.　Metabolomics　analysis　revealed　zeolite　powder　addition　enhanced　the　metabolisms　of　amino　acids,　nicotinate　and　porphyrin　through　increasing　glutamate　content,　and　improved　EPS　secretion,　heme　c　content　and　particle　size.　Besides,　high　ammonia　enriched　by　zeolite　powder　was　conducive　to　improve　anammox　activity　and　NRR.　This　study　provides　the　metabolic　insights　into　the　mechanism　of　zeolite　powder　enhancing　SPDA,　which　is　meaningful　towards　overcoming　the　limitations　in　practical　application　of　SDPA.