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As a highly toxic pollutant, phenol is typically present in some high-strength nitrogenous wastewater. In this study, a synthetic coking wastewater with 400 mg L-1 ammonia-nitrogen and 50-250 mg L-1 phenol was treated. Results showed that simultaneous carbon oxidation, partial nitritation, denitritation and anammox (SCONDA) was successfully achieved by step-wise phenol addition. At 200 mg L-1 phenol, 99.8% phenol, 97.5% COD and 89.8% nitrogen could be together removed. However, further increase in phenol concentration caused significant deterioration of the short-terms nitrogen removal efficiency. High-throughput sequencing revealed remarkable evolution in microbial biodiversity, community composition, especially functional species at different phenol concentrations. When the phenol addition was increased from 200 to 250 mg L-1, the relative abundance of Candidates Kuenenia as predominant anammox species decreased by 87.1%, while phenol-degrading bacteria was increasingly abundant. Furthermore, the removal mechanism of phenol and nitrogen was elucidated by the collaboration among different key functional microbial consortia. (C) 2019 Elsevier Ltd. All rights reserved.
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