The　effluents　of　municipal　wastewater　treatment　plant　(WWTP)　contain　excessive　nitrogen　and　phosphorus　compared　with　the　concentration　in　rivers　or　lakes.　To　reduce　the　pollutant　load　placed　on　aqueous　environments,　constructed　wetland　(CW)　technology　has　been　widely　applied　to　advanced　wastewater　treatment.　Packing　substrates　in　CW　could　remove　various　pollutants.　Steel　slag,　yellow　earth,　kaolin,　volcanic　rock,　anthracite,　and　ceramsite　could　effectively　remove　phosphorus　(P);　volcanic　rock,　ceramsite,　zeolite,　yellow　earth,　manganese　sand,　and　activated　carbon　have　an　affinity　for　ammonia　nitrogen　(NH4+-N).　After　24　h　reactions　with　the　WWTP　standard　1B　synthetic　wastewater,　four　packing　substrates,　i.e.,　volcanic　rock　and　anthracite　(1:1),　volcanic　rock　and　yellow　earth　(2:1),　zeolite　and　yellow　earth　(2:1),　and　manganese　sand　and　activated　carbon　(1:3),　could　remove　over　56%　and　30%　of　NH4+-N　and　phosphorus　respectively.　In　addition,　anthracite　and　volcanic　rock　(1:3),　anthracite　and　activated　carbon　(1:40),　anthracite　and　manganese　sand　(1:5),　and　anthracite　and　zeolite　(1:4)　effectively　purified　NH4+-N　and　phosphorus　in　secondary　WWTP　effluent,　with　removal　efficiency　exceeding　39%　and　27%,　respectively.　A　sequential　experiment　was　performed　to　optimize　packing　substrates　ratios　in　CW　with　volcanic　rock　and　anthracite,　ceramsite　and　yellow　earth,　and　manganese　sand　and　activated　carbon.　When　the　quantity　of　the　substrate　was　doubled,　most　packing　substrates　adsorb　more　than　50%　phosphorus　and　NH4+-N　of　the　standard　1B　WWTP　synthetic　wastewater.　Considering　the　removal　efficiency　of　packing　substrates　on　phosphorus　and　NH4+-N,　it　is　suggested　that　manganese　sand　and　activated　carbon　(1:3),　volcanic　rock　and　anthracite　(2:1),　and　yellow　earth　are　appropriate　substrates　for　CW　in　WWTP　effluent　advanced　treatment.