Human　papillomavirus　(HPV)　infection　was　associated　with　some　carcinomas,　especially　malignant　tumors　in　upper　digestive　tract,　upper　respiratory　tract,　and　genitourinary　system.　The　mechanism　of　the　viral　transformation　of　normal　cells　is　still　not　very　clear.　To　investigate　the　tumorigenesis　of　epithelial　cells,　E6/E7-induced　malignant　transformation　model　cells　were　used　for　expression　profiling　analysis　by　performing　RNA　expression　microarray　detection.　Bioinformatics　analysis　was　applied　to　investigate　the　cellular　process　changes　along　with　the　E6/E7　expression　in　SHEE　cells.　The　differentially　expressed　genes　were　further　grouped　and　uploaded　for　Search　Tool　for　the　Retrieval　of　Interacting　Genes　analysis.　The　protein-protein　interaction　results　were　visualized.　The　hub　genes　and　their　first-neighbors　genes　were　selected,　followed　by　gene　ontology　and　Kyoto　Encyclopedia　of　Genes　and　Genomes　pathway　analysis.　The　obtained　results　demonstrated　that　tumor-related　biological　processes　began　to　emerge　during　the　carcinogenesis　process　from　48th　passage　to　76th　passage　of　SHEE　cells　after　E6/E7　expression.　Ten　hub　genes　were　identified　and　analyzed　during　the　E6/E7-induced　tumorigenesis.　This　study　explores　the　gene　expression　network　in　the　progressive　transformation　of　immortalized　esophageal　epithelial　cells　induced　by　E6/E7　expression.　Understanding　the　biological　processes　and　hub　genes　that　first　appear　during　the　transformation　will　provide　some　clues　to　the　mechanism　of　E6/E7-induced　carcinogenesis　of　esophageal　epithelial　cells.