Secondary　effluents　as　one　of　the　main　＇hotspots＇　of　spreading　antibiotic　resistance　genes　(ARGs)　into　the　environment　have　attracted　considerable　attentions.　However,　conventional　disinfection　methods　may　be　inadequate　for　the　successful　removal　of　ARGs.　Therefore,　this　study　investigated　the　removal　efficiency　and　the　regeneration　potential　of　ten　ARGs　in　secondary　effluent　using　sodium　persulfate　activated　by　Ginkgo　biloba　L.　modified　nanoscale　zero-valent　iron.　Moreover,　the　role　of　bacterial　community　was　not　yet　clear　when　ARGs　were　removed　by　persulfate　treatment　(PT).　Therefore,　quantitative　PCR　and　Illumina　MiSeq　sequencing　were　applied　for　further　exploration.　The　results　depicted　that　98.6%　bacterial　16S　rRNA　gene　was　removed　within　10　min.　After　0.5　h　PT,　the　removal　efficiency　of　target　ARGs　decreased　in　the　following　order:　Tn916/1545　=　aac　(below　the　limit　of　detection)　＞　int　I1　(99.99%)　＞　tet　E　(99.64%)　＞　mex　F　(99.10%)　＞　tet　W　(94.57%)　＞　qnr　S　(90.18%)　＞　van　G　(82.21%)　＞　bla-TEM　(64.15%)　＞　cat　A1　(23.13%).　Even　upon　after　48　h　storage,　ARGs　abundances　were　stable.　The　influence　of　pH　on　ARGs　removal　efficiency　was　not　significant.　The　results　of　heatmap　and　principal　coordinate　analysis　depicted　that　the　abundance　of　ARGs　did　not　increase　with　the　changes　of　community　structure.　This　study　revealed　PT　as　an　effective　method　could　reduce　abundance　of　ARGs.　The　ARGs　carried　by　bacteria　did　not　multiply　in　their　hosts　and　pass　on　to　other　bacterial　populations　further.