The　purpose　of　this　study　was　to　rapidly　improve　the　denitrification　packing　activity　in　the　denitrification　pond　sludge　of　sewage　plants,　to　realize　the　engineering　application　of　embedding　immobilization,　and　to　explore　the　characteristics　of　the　microbial　community　of　the　embedding　packing.　The　effects　of　different　C/N　ratios,　temperature,　and　pH　on　the　activity　of　embedding　filler　were　studied　by　batch　experiments,　and　the　biological　community　characteristics　of　embedding　filler　were　studied　by　high-throughput　sequencing.　Results　showed　that　the　embedding　packing　in　C/N　to　10,　the　temperature　of　30,　and　pH　of　7.5±0.3,　resumed　5.37　mg•(g•h)-1　of　the　initial　activity　after　7　days.　Under　the　optimized　culture　conditions　of　C/N　ratio　of　10,　temperature　of　25,　and　pH　of　8.0,　the　specific　rate　of　denitrification　can　be　increased　by　15　times　to　80.17　mg•(g•h)-1　after　15　days,　and　stabilized　at　that　level.　SEM　results　showed　that　there　were　a　large　number　of　mass　transfer　channels　inside　the　embedding　fillers,　and　that　the　bacteria　grew　well　as　clusters　in　the　encapsulated　fillers.　The　high　throughput　sequencing　showed　that　the　denitrifying　function　of　Thauera　and　Thermomonas　were　24.27%　and　8.23%　respectively,　which　ensured　the　high　efficiency　of　denitrifying　nitrogen　removal.　The　rapid　proliferation　of　Thauera　dominant　bacteria　and　Thermomonas　under　optimal　culture　conditions　was　the　main　reason　for　the　rapid　increase　of　packing　activity.　©　2018,　Science　Press.　All　right　reserved.