SERS　and　SEF　technology　with　sliver　nanoparticles　can　achieve　very　high　sensitivity,　thus　it　can　be　used　to　analysis　biological　samples.　Here,　a　technique　was　reported,　which　can　be　used　to　improve　detection　results　of　SYBR　Green　by　SERS　and　SEF　at　the　same　time　by　using　silver　nanoparticles　in　microchannels.　The　fundamental　and　enhancement　effect　of　this　method　were　studied.　First,　the　excimer　laser　was　used　to　write　directly　on　the　PMMA　substrate　to　etch　the　microchannel　with　the　width　of　200　μm　and　depth　of　68　μm.　The　prepared　silver　precursor　solution　was　then　added　to　the　microchannel.　The　surface　enhanced　Raman(SERS)　and　surface　enhanced　fluorescence(SEF)　substrate　were　prepared　by　heating.　Next,　the　Raman　and　fluorescent　signals　were　compared　with　and　without　adding　silver　nanoparticles.　Besides,　the　effect　of　silver　nanoparticles　on　the　Raman　and　fluorescent　signal　enhancement　of　SYBR　GREEN　I　in　micro-channel　was　studied.　The　results　show　that　the　enhancement　factor　of　the　surface-enhanced　Raman(SERS)　experiment　with　silver　nanoparticles　is　3.5×103.　The　intensity　of　the　fluorescence　signal　of　the　samples　with　silver　nanoparticles　increases　by　about　1　time　compared　with　that　of　the　fluorescent　signal　without　silver　nanoparticles.　When　SYBR　Green　is　detected　in　the　microchannel,　it　significantly　increases　the　Raman　and　fluorescent　signals　by　adding　silver　nanoparticles.　It　can　be　used　in　microfluidic　chip　technology　with　SYBR　Green　dye.　©　2018,　Science　Press.　All　right　reserved.