The　effects　of　low　substrate　ratio,　cooling　methods,　and　pH　on　nitrogen　removal　performance　were　studied　in　a　laboratory-scale　anaerobic　ammonium　oxidation　reactor　(ASBR)　while　treating　simulated　domestic　waste　water.　The　results　illustrated　that　the　average　removal　efficiencies　of　NH4+-N　and　NO2--N　increased　from　54.4%　and　65.3%　to　95.8%　and　92.5%,　respectively,　at　a　temperature　of　30and　an　influent　concentration　of　NO2--N　of　(30±0.2)mg•L-1.　The　substrate　ratio　(NO2--N/NH4+-N)　increased　from　0.9　to　1.4.However,　the　removal　efficiency　of　NH4+-N　was　affected　negligibly,　and　the　average　removal　efficiency　of　NO2--N　decreased　to　54.6%　when　the　substrate　ratio　was　increased　to　1.6,　suggesting　that　the　nitrogen　removal　performance　of　anaerobic　ammonium　oxidation　was　best　when　the　substrate　ratio　was　close　to　the　theoretical　value　of　1.32.The　average　removal　efficiencies　of　NH4+-N　and　NO2--N　decreased　from　97.5%　and　98.5%　to　35.2%　and　40.1%,　respectively,　when　the　temperature　of　the　reactor　dropped　from　30　to　15　at　one　time.　The　average　removal　efficiencies　of　NH4+-N　and　NO2--N　dropped　from　97.7%　and　98.6%　to　52.7%　and　62.4%,　respectively,　when　the　ladder　cooling　method(30FY125FY120FY115)was　used.　The　average　removal　efficiencies　of　NH4+-N　and　NO2--N　increased　initially　and　then　decreased　when　the　pH　was　increased　gradually　from　7.7　to　8.5.The　highest　nitrogen　removal　efficiency　was　achieved　when　the　pH　was　controlled　at　8.3　with　a　substrate　ratio　of　NO2--N/NH4+-N　equal　to　1.4.　©　2018,　Science　Press.　All　right　reserved.