Lab-scale　simultaneously　anaerobic　ammonia　oxidation　and　denitrification　(SAD)　process　was　operated　in　Municipal　Waste　Water　Treatment　Plant　(WWTP).　Sewage　treated　by　A/O　and　nitrification　process　was　served　as　substance　to　start　up　anaerobic　ammonia　oxidation　filter　reactor.　Adding　organic　carbon-source　to　influent　was　served　as　substance　to　start　up　SAD　filter　reactor　after　successful　start-up　of　Anammox　reactor.　Glucose　was　selected　as　organic　carbon-source　because　it　is　harmless　and　inexpensive.　The　result　show　that　SAD　process　performed　well　with　10,　20,　and　30　mg/L　Glucose　added　to　effluent　sewage　at　ambient　temperature　and　average　total　nitrogen　concentration　of　effluent　was　9.16,　8.10,　and　6.41　mg/L.　Compared　with　Anammox　process,　SAD　process　performed　better　and　total　nitrogen　concentration　in　effluent　decreased　16-42　percents.　Stability　of　SAD　process　was　destroyed　and　SAD　process　turned　to　denitrification　process　when　30　mg/L　Glucose　was　added　in　influent　sewage　in　winter.　Ammonia　in　effluent　increased　from　0.5　mg/L　to　6.2　mg/L.　The　results　indicate　that　temperature　has　superior　effect　on　SAD　process.　The　total　nitrogen　concentration　in　effluent　varied　from　6.5　to　8.5　mg/L　when　concentration　of　Glucose　decreased　to　20　mg/L.　In　winter,　the　effluent　of　SAD　process　reached　the　1A　level　of　integrated　discharge　standard　of　water　pollutants　applied　in　Beijing　City.　©　2018,　Editorial　Board　of　Journal　of　Harbin　Institute　of　Technology.　All　right　reserved.