A　lab-scale,　completely　anaerobic　ammonium　oxidation　(ANAMMOX)　process　was　operated　in　a　municipal　wastewater　treatment　plant　(WWTP).　Sewage　effluent　treated　by　an　A/O　process　and　nitrification　process　was　input　as　the　substance　to　start　up　the　up-flow　ANAMMOX　filter　reactor.　After　the　109th　day,　the　ammonia　removal　rate　and　nitrite　removal　rate　were　greater　than　90%　for　15　successive　days　and　the　nitrogen　removal　rate　was　higher　than　70%.　The　ANAMMOX　filter　reactor　successfully　started　up.　From　days　245　to　333,　the　reactor　was　running　during　the　winter.　The　weight　of　biomass　reached　12.24　mg•g-1,　and　the　average　nitrogen　removal　rate　was　54.3%.　Backwash　was　adopted　at　day　461,　and　the　weight　of　biomass　decreased　to　8.01　mg•g-1.　From　days　605　to　693,　the　reactor　was　running　in　the　winter　again.　The　weight　of　biomass　was　10.41　mg•g-1,　and　the　average　nitrogen　removal　rate　was　sustained　at　69.7%.　Compared　with　the　previous　winter,　the　weight　of　biomass　was　lighter　but　the　total　nitrogen　removal　loading　was　23%　greater.　For　the　entire　operation,　the　ANAMMOX　rate　at　high　temperature　was　stable　but　that　at　low　temperature　increased　from　1.5　kg•(kg•d)-1　to　3.6　kg•(kg•d)-1.　The　results　show:　Long-term　domestication　at　low　temperature　was　in　favor　of　improving　treatment　efficiency　of　ANAMMOX　process　in　cold　environment　and　realized　ANAMMOX　process　operated　efficiently　in　winter.　©　2018,　Science　Press.　All　right　reserved.