In　the　progress　of　urbanization,　serious　crashes　on　curved　sections　of　urbanization　highway　are　a　significant　problem.　The　main　reason　is　that　urbanization　highways　have　an　impact　on　drivers＇　physiological　status.　In　this　paper,　three　road　simulation　scenarios　were　implemented　in　a　driving　simulator:　＇non-urbanization　highway＇,　＇semi-urbanization　highway＇　and　＇urbanization　highway＇.　Drivers＇　physiological　features　were　studied　by　observing　their　heart　rate　growth　rates.　The　results　demonstrate　that　heart　rate　growth　rate　was　significantly　affected　by　the　degree　of　urbanization　of　the　highway.　On　curved　segments　of　urbanization　highway,　heart　rate　growth　rate　changes　and　vehicle　speed　fluctuations　were　significant.　Moreover,　heart　rate　growth　rates　in　the　uplink　direction　of　curves　were　higher　than　those　in　the　downlink　direction.　Speed　and　heart　rate　growth　rate　were　highly　correlated.　Within　the　same　degree　of　urbanization　highway,　drivers＇　heart　rate　growth　rates　first　decreased　and　then　increased　with　increasing　speed.　A　correlation　model　of　the　degree　of　urbanization,　vehicle　speed　and　heart　rate　growth　rates　is　proposed　and　used　to　derive　safety　thresholds　based　on　heart　rate　growth　rate.　The　safety　thresholds　of　heart　rate　growth　rate　were　7.6%,　22.0%　and　37.0%　at　＇good＇,　＇fair＇　and　＇poor＇　levels,　respectively.　The　main　results　provide　a　basis　for　identifying　dangerous　road　sections　and　improving　the　driving　comfort　and　safety　of　urbanization　highways.