The　development　of　low-cost,　stable,　and　recyclable　multiphase　catalysts　in　advanced　oxidation　process　is　essential　for　efficient　degradation　of　antibiotics.　In　this　study,　a　BC-Co-W-700　catalyst　was　used　to　activate　peroxymonosulfate　(PMS)　for　chlortetracycline　(CTC)　degradation.　The　catalyst　was　prepared　using　reclaimed　biomass　material　as　a　matrix.　The　biochar-based　catalyst　possessed　abundant　active　sites　and　realized　the　in-situ　growth　of　Co　and　CoWO4.　The　BC-Co-W-700/PMS　achieved　100%　degradation　of　CTC　(20　mg/L)　within　18　min.　Quenching　and　electron　paramagnetic　resonance　experiments　were　conducted　to　investigate　the　reactive　oxygen　species　(ROS)　in　the　BC-Co-W-700/PMS　system.　The　identified　ROS　in　this　system　included　1O2,　O2　center　dot　,　SO4　center　dot　,　and　center　dot　OH.　Abundant　ROS　endowed　the　system　with　strong　degradation　performance,　applicability,　and　anti　-interference　ability.　The　catalytic　mechanism　of　BC-Co-W-700/PMS　system　was　comprehensively　investigated　using　a　combination　of　electrochemical　experiments　and　theoretical　calculations.　The　in-situ　pyrrolic　N　formed　by　biochar　had　a　strong　adsorption　capacity　for　CTC,　allowing　pollutants　to　quickly　adsorb　to　the　catalyst　surface.　This　greatly　shortened　the　distance　required　for　the　catalytic　reaction.　The　introduction　of　W　led　to　abundant　oxygen　vacancies　on　BC-Co-W-700,　causing　the　catalyst　to　produce　a　large　amount　of　1O2.　Furthermore,　the　oxygen　vacancies　provided　a　high　concentration　of　electrons　for　the　conversion　of　Co3+/Co2+.　In　addition,　CTC　degradation　pathways　were　analyzed　using　density　functional　theory　calculations,　and　the　toxicity　of　the　in-termediates　was　evaluated　using　the　quantitative　structure-activity　relationship　combined　with　experiments.　The　green　catalyst　synthesis　method　proposed　in　this　paper　could　provide　a　new　approach　for　the　efficient　degra-dation　of　antibiotics　in　wastewater.