Non-enzymatic　electrodes　based　on　noble　metals　have　excellent　selectivity　and　high　sensitivity　in　glucose　detection　but　no　such　shortcomings　as　easy　to　be　affected　by　pH,　temperature,　and　toxic　chemicals.　Herein,　spherical　gold-nickel　nanoparticles　with　a　core-shell　construction　(Au@Ni)　are　prepared　by　oleylamine　reduction　of　their　metal　precursors.　At　an　appropriate　Au/Ni　ratio,　the　core-shell　Au@Ni　nanoparticles　as　a　sensor　for　glucose　detection　combine　the　high　electrocatalytic　activity,　good　selectivity　and　biological　compatibility　of　Au　with　the　remarkable　tolerance　of　Ni　for　chlorine　ions　(Cl-)　and　poisoning　intermediates　in　catalytic　oxidation　of　glucose.　This　electrode　exhibits　a　low　operating　voltage　of　0.10　V　vs.　SCE　for　glucose　oxidation,　leading　to　higher　selectivity　compared　with　other　Auand　Ni-based　sensors.　The　linear　range　for　the　glucose　detection　is　from　0.5　mmol　L-1　to　10　mmol　L-1　with　a　rapid　response　time　of　ca.　3　s,　good　stability,　sensitivity　estimated　to　be　23.17　mu　A　cm(-2)　mM(-1),　and　a　detection　limit　of　0.0157　mM.　The　sensor　displays　high　anti-toxicity,　and　is　not　easily　poisoned　by　the　adsorption　of　Cl-　in　solution.