High-efficiency　denitrifying　bacteria　can　improve　wastewater　treatment　efficiency.　In　order　to　rapidly　culture　high-efficiency　denitrifying　bacteria,　batch　trials　were　conducted　at　different　carbon　sources　(No.　1　and　No.　2　fermented　liquid)　and　NO3--N　concentration　gradients　(30　mg·L-1-150　mg·L-1-300　mg·L-1).　The　results　showed　that　No.　2　fermented　liquid　could　finish　high-efficiency　denitrifying　bacteria　[300　mg·(L·h)-1]　cultivation　in　11　d,　which　is　17　d　before　No.1　fermented　liquid　could.　At　the　same　time,　high-efficiency　denitrifying　bacteria　could　effectively　remove　ammonia-nitrogen　and　total　phosphorus,　and　maximum　removal　rates　were　34.43　mg·(L·h)-1　and　2.98　mg·(L·h)-1,　respectively.　Furthermore,　high-throughput　sequencing　was　employed　to　characterize　and　compare　the　community　structure　and　diversity.　The　species　abundance　and　diversity　were　reduced　but　the　category　and　proportion　of　denitrifying　bacteria　were　increased.　In　addition,　bacteria　composition　and　quantity　changed　after　cultivation　and　Thauera　and　Pseudomonas　were　the　dominant　contributors　to　the　effective　removal　of　nitrate　in　the　SBR(sequencing　batch　reactor).　Denitrifying　phosphorus　accumulating　bacteria　(Rhodocyclaceae　and　Pseudomonadaceae)　and　heterotrophic　nitrifying　bacteria　(Pseudomonas,　Alcaligenes,　Bacillus,　and　Comamonas)　proved　the　capacity　of　the　system　to　remove　ammonia-nitrogen　and　total　phosphorus.　©　2017,　Science　Press.　All　right　reserved.