The　inhibition　effect　of　three　different　kinds　of　antibiotics　on　the　nitrogen　removal　performance　of　anammox　granules　was　investigated　by　serum　bottle　batch　tests.　The　antibiotics　used　in　the　experiment　were　penicillin　G　sodium,　oxytetracycline　HCl　and　polymyxin　E　sulphate,　which　performed　different　inhibition　mechanisms.　As　a　result,　when　the　batch　test　was　conducted　without　the　addition　of　antibiotic,　the　NH4+-N　and　NO2--N　removal　rates　of　the　anammox　granules　were　0.252　and　0.375kg　N/(kg　VSS·d),　respectively.　Besides,　the　anammox　activity　of　the　granules　was　not　inhibited　with　the　penicillin　G　sodium　concentration　of　3000mg/L.　However,　the　anammox　activity　of　the　granules　was　largely　inhibited　by　oxytetracycline　HCl　and　polymyxin　E　sulphate.　With　the　oxytetracycline　HCl　concentrations　of　50,　100,　150,　200　and　400mg/L,　the　NH4+-N　removal　rates　were　0.250,　0.237,　0.200,　0.117　and　0.062kg　N/(kg　VSS·d),　respectively;　The　NO2--N　removal　rates　were　0.324,　0.304,　0.296,　0.244　and　0.069kg　N/(kg　VSS·d),　respectively.　Meanwhile,　with　the　polymyxin　E　sulphate　concentrations　of　30,　70,　90,　100　and　300mg/L,　the　NH4+-N　removal　rates　were　0.230,　0.134,　0.094,　0.022　and　0.007kg　N/(kg　VSS·d),　respectively;　The　NO2--N　removal　rates　were　0.351,　0.203,　0.133,　0.039　and　0.004kg　N/(kg　VSS·d),　respectively.　The　inhibition　effects　of　the　three　kinds　of　antibiotics　were　in　the　order　as　follows:　polymyxin　E　sulphate＞oxytetracycline　HCl＞penicillin　G　sodium.　©　2017,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.